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Image Search Results
Journal: Frontiers in Molecular Neuroscience
Article Title: Global Gene Knockout of Kcnip3 Enhances Pain Sensitivity and Exacerbates Negative Emotions in Rats
doi: 10.3389/fnmol.2019.00005
Figure Lengend Snippet: Target genes modulated by transcriptional repressor KChIP3/DREAM.
Article Snippet: For Western blot,
Techniques:
Journal: Frontiers in Molecular Neuroscience
Article Title: Global Gene Knockout of Kcnip3 Enhances Pain Sensitivity and Exacerbates Negative Emotions in Rats
doi: 10.3389/fnmol.2019.00005
Figure Lengend Snippet: Validation of Kcnip3 gene knockout in Kcnip3 -/- rats by quantitative real-time PCR (qPCR) and Western blot. (A) qPCR analysis of Kcnip3 gene expression in the cerebral cortex of wild-type (WT) and Kcnip3 -/- rats. ∗∗ p < 0.01, paired t -test. (B) Western blot analysis of KChIP3 protein expression in the central nervous system of wild-type and Kcnip3 -/- rats. KO, knockout; Hippo, hippocampus. (C) Western blot analysis of KChIP1, 2 and 4 expression in the peripheral and central nervous system of wild-type and Kcnip3 -/- rats under normal condition. DRG, dorsal root ganglion. (D) Relative quantification of KChIP1, 2 and 4 protein levels in the peripheral and central nervous system of wild-type and Kcnip3 -/- rats. n = 3 for both groups. ∗ p < 0.05, paired t -test.
Article Snippet: For Western blot,
Techniques: Gene Knockout, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Knock-Out
Journal: bioRxiv
Article Title: A urine-dependent human urothelial organoid offers a promising alternative to rodent models of infection
doi: 10.1101/152033
Figure Lengend Snippet: (a) 3D confocal model and (b) orthogonal reslice from a 100 slice Z-stack of HBEP organoid. The HBEP organoid exhibited the correct spatial expression of Cytokeratin-20 (CK20, umbrella cells, magenta) and Cytokeratin-8 (CK8, throughout urothelium, green). (c) 3D confocal model and (d) orthogonal reslice from a 120 slice Z-stack of HBLAK organoid. As with the primary HBEP mimetic, the HBLAK organoid also expressed CK20 (magenta) at the apical surface and CK8 (green) throughout the tissue. (e-h) Single optical slices at apical region of HBEP (e,f) and HBLAK (g,h) organoids with corresponding orthogonal cross sections shown directly beneath. (e,g) Expression of muscarinic receptor 2 (M2, green) and muscarinic receptor 3 (M3, magenta). Both receptors were found throughout the tissue in both models. Phalloidin-stained F-actin is presented in grey. (f,h) Expression of Uroplakin-III (UP3, red) preferentially at apical region of both models. Phalloidin-stained F-actin is presented in grey. (i) High-power single optical slice of UP3 (red) expression in HBEP model. (j) Pattern of UP3 (red) expression found in an exfoliated urothelial cell harvested from a chronic UTI patient. (k) High-power single optical slice of UP3 (red) expression in HBLAK model. (l) Further example of the pattern of UP3 (red) expression found in patient-isolated urothelial cells. DAPI-stained DNA is presented in grey. Scale bars represent 20μm.
Article Snippet: Tissue was incubated overnight at 4°C with primary antibodies in PBS containing 1% NGS as follows: 1:10 dilution of mouse anti-uroplakin-III (UP3) monoclonal antibody (clone AU1, 651108, Progen Bioteknik); 1:50 dilution of mouse anti-Cytokeratin 8 (CK8) monoclonal antibody (clone H1, MA1-06317, Thermo Fisher); 1:100 dilution of
Techniques: Expressing, Staining, Isolation